Isolation of the terpestacin from the endophytic fungus Drechslera ravenelii

Studies about biprospection in endophytic fungi isolated from Smalanthus sonchifolius, the fermentation in solid medium rice-oat by 720 hours of the fungus Drechslera ravenelii (SS33) led to the isolation of sesterpene terpestacin. The molecule terpestacin has attracted the scientific community attention due to inhibitory activity in the syncytium formation caused by the HIV virus and also by presenting unusual chemical structure. After fermentation in the rice-oat medium, the hydroalcoholic extract was submitted to the liquid-liquid partition with n-hexane, dichloromethane, ethyl acetate and butanol. The dichlorometanic fraction was subjected to chromatographic processes to obtain 19.0 mg of terpestacin. The structure of this compound was were elucidated by detailed spectroscopic analyses. This study reported the isolation of terpestacin from the endophytic fungus Drechslera ravenelii for the first time.
Eliane O. Silva, Willian J. Andrioli, Adriana A. Lopes, Monica T. Pupo, Jairo K. Bastos. Isolation of the terpestacin from the endophytic fungus Drechslera ravenelii. International Journal of Nanobiotechnology and Pharmacy. 2013. N2. p.2-7.
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